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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, DB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1462 |
| Description | Anti-phospho LSD1 (Ser 112) Antibody |
| Alternate Names |
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| Background Information | Lysine-specific histone demethylase 1A (UniProt Q6ZQ88; also known as BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2) is encoded by the Kdm1a (also known as Aof2, Kiaa0601, Lsd1) gene (Gene ID 99982) in murine species. The FAD+-dependent, lysine-specific demethylase (LSD) LSD1 is a component of the REST corepressor (CoREST) complexes that mediate gene repression by catalyzing the demethylation of mono- and di-methylated histone H3 Lys4 (H3K4me1/2). LSD1 is also involved in gene activation process associated with nuclear receptors through demethylation of H3K9me1/2. In addition, LSD1 is known to demethylate non-histone substrates, such as p53 and Dnmt1. LSD1 is reported to be phosphorylated on Ser112 by PKCalpha and thereby mediate PKCalpha signaling-dependent circadian clock regulation. Ser112 phosphorylation induces LSD1 interaction with CLOCK:BMAL1 to facilitate E-box-dependent transcriptional activation. Knockin mice bearing Lsd1(SA/SA) alleles coding for phosphorylation-defective S112A LSD1 mutant are reported to exhibit altered circadian rhythms in locomotor behavior with attenuated rhythmic expression of core clock genes and impaired circadian clock phase resetting. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho LSD1 (Ser 112) Antibody is an antibody against phospho LSD1 (Ser 112) for use in Western Blotting, DOT Blot. |
| Key Applications |
|
| Application Notes | DOT Blot Analysis: A representative lot detected the immunogen peptide with phosphorylated Ser112 (equivalent to human pSer111), but not the corresponding peptide with non-phosphorylated Ser112 (Nam, H.J., et Al. (2014). Mol. Cell 53:791-805). Western Blotting Analysis: A representative lot detected phosphorylation induction of exogenously expressed human LSD1 upon PMA treatment of 24-hour starved HEK293T transfectants. Phosphorylated LSD1 was detected in the nuclear, but not cytosolic fraction and PKC inhibitor Go 6976 (Cat. No. 365250) co-treatment prevented PMA-induced LSD1 phosphorylation (Nam, H.J., et Al. (2014). Mol. Cell 53:791-805). Western Blotting Analysis: A representative lot detected a time-dependent LSD1 phosphorylation level in liver extracts from mice kept in a constantly dark (DD) cycle. Nuclear extracts from MEFs at different time points following circadian gene transcription induction by dexamethasone treatment likewise showed circadian-like oscillation of LSD1 phosphorylation level (Nam, H.J., et Al. (2014). Mol. Cell 53:791-805). |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to a mouse LSD1 sequence containing the phosphorylated Ser112. |
| Epitope | pSer112. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | The Ser112 designation is based on murine KDM1A/LSD1 sequence (UniProt Q6ZQ88). Murine Ser112 is equivalent to human Ser111 and rat Ser106 (UniProt O60341 & F1MA31). Specificity of this polyclonal antibody was confirmed by DOT blot analysis using the immunogen peptide and the corresponding non-phosphorylated peptide, as well as by Western blotting analysis of PMA-induced target phosphorylation, lambda phosphatase treatment of the cell lysate prior to Western blotting completely abolished the target band detection (Nam, H.J., et Al. (2014). Mol. Cell 53:791-805). |
| Species Reactivity |
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| Species Reactivity Note | Mouse, Human. Predicted to react with Rat, Porcine, Rhesus Macaque, Zebrafish based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~115 kDa observed. 92.90/95.16 kDa (human isoform 1/2), 92.85 (mouse), and 94.60 kDa (rat) calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human LSD1-overexpressing HEK293T cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected PMA-induced phosphorylation of exogenously expressed human LSD1 in 24-hour starved HEK293T transfectants. Lambda phosphatase treatment of the membrane prior to antibody probing completely abolished the target band detection. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |