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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IP, WB, ChIP-seq, ChIP | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1934 |
| Description | Anti-Gcn5 Antibody |
| Alternate Names |
|
| Background Information | Histone acetyltransferase KAT2A (EC 2.3.1.48; UniProt Q92830; also known as General control of amino acid synthesis protein 5-like 2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, Lysine acetyltransferase 2A, STAF97) is encoded by the KAT2A (also known as GCN5, GCN5L2, HGCN5) gene (Gene ID 2648) in human. Histone acetyltransferases (HATs) are transcription co-activators recruited by transcriptional activators to target gene promoters, where they enhance the transcription activity by acetylating lysine residues within the N-terminal regions of histones. KAT2A/KAT2B, MYST (MOZ, Ybf2 (Sas3), Sas2, and Tip60), TAFII250, CREBBP/EP300, and SRC represent the five HAT families. In addtion to functioning as transcription co-activators, KAT2A (GCN5) and KAT2B (PCAF) are also reported to repress IFN‐β production and innate antiviral immunity by inhibiting the innate immune signaling kinase TBK1 in the cytoplasm in a HAT‐independent and non‐transcriptional manner. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Gcn5 Antibody is an antibody against Gcn5 for use in Immunoprecipitation, Western Blotting, ChIP-seq, Chromatin Immunoprecipitation (ChIP). |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.2 µg/mL from a representative lot detected Gcn5 immunoprecipited from HeLa nuclear extract. Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot immunoprecipitated KAT2A/GCN5-associated DNA fragments by ChIP using chromatin preparations from murine CH12 and human GM12878 lymphoblasts (Cheng, Y., et Al. (2014). Nature. 515(7527):371-375). ChIP-seq Analysis: A representative lot was employed to generate Gcn5 genome-wide binding profiles by ChIP-seq analysis using chromatin preparations from murine CH12 and human GM12878 lymphoblasts (Cheng, Y., et Al. (2014). Nature. 515(7527):371-375). |
| Biological Information | |
|---|---|
| Immunogen | Ovalbumin-conjugated linear peptide corresponding to the N-terminal sequence of human Gcn5. |
| Epitope | N-terminus. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody was raised against human KAT2A isoform GCN5-L N-terminal sequence that is 95% conserved in murine and rat Kat2a/Gcn5, but missing in human spliced isoform GCN5-S (UniProt Q92830-2). |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| Molecular Weight | ~100 kDa observed. 93.93 kDa (human GCN5-L; UniProt Q92830-1) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunoprecipitation in HeLa nuclear extract. Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipited Gcn5 from 500 µg of HeLa nuclear extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |