Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE217 |
| Description | Anti-CUX1 Antibody, a.a. 861 |
| Alternate Names |
|
| Background Information | Homeobox protein cut-like 1 (CUX1) is also known as CCAAT displacement protein (CDP) and Homeobox protein cux-1. CUX1 is thought to repress expression of developmentally regulated genes involved in mammalian development, likely through the prevention of CCAAT factor binding at promoters. CUX1 also represses T-cell receptor (TCR) beta enhancer function by binding to matrix attachment region beta (MARbeta), and is a component of the nf-munr repressor, which binds to matrix attachment regions (MARs) of the immunoglobulin heavy chain enhancer. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Cux1 using this rabbit polyclonal antibody, Anti-CUX1 Antibody, a.a. 861 validated for use in western blotting & IP. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 1.25 µg from a representative lot immunoprecipitated CUX1 in 500 µg of HeLa nuclear extract. Alexa Fluor® is a registered trademark of Life Technologies®. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to a region of amino acids beginning at 861 of human CUX1. |
| Epitope | Region of amino acids beginning at 861 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the region of amino acids beginning at 861 of CUX1. This antibody is expected to cross react with all known isoforms of CUX1. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Other homologies: Mouse (95% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~200 kDa and ~110 kDa observed. Uniprot describes 11 different isoforms. A full length protein has been described at 200 kDa and is proteolytically cleaved into the p150, p110, p90, and p80 isoforms. Additionally, the p75 isoform is the result of alternative splicing (Sansregret, L, et Al. (2008). Gene. 412(1-2):84-94.). Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa nuclear extract. Western Blotting Analysis: 0.25 µg/mL from a representative lot detected CUX1 in 10 µg of HeLa nuclear extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |