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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ChIP | Sh | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE23 |
| Description | Anti-Gdown1 Antibody |
| Alternate Names |
|
| Background Information | Gdown1 (DNA-directed RNA polymerase II subunit GRINL1A; DNA-directed RNA polymerase II subunit M; glutamate receptor-like protein 1A) is a nuclear protein belonging to the GRINL1 family. It binds to the RNA Polymerase II (G) molecule (RNAPII (G)) and prevents its interaction with transactivating factors, thereby suppressing RNAPII (G)-mediated transcriptional events. Gdown1 also indirectly suppresses RNAPII (G)-transcription by blocking the binding of the elongation-enhancing factor TFIIF to the elongation complex. Gdown1 also prevents TFIIF-mediated assembly of the preinitiation complex required for recruiting RNAPII to the promoter region, by binding competitively to Pol II at the RPB1 and RPB5 subunits recognized by TFIIF. Gdown1 may also inhibit the termination factor TTF2. The effects of Gdown1 are blocked by the P-TEFb. Overall, Gdown1 regulates the transcriptional activity of RNAPII (G) at multiple promoter regions by various mechanisms. Gdown1 has been detected in brain, testis, prostate, lung, small intestine, heart, kidney, and skeletal muscle. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified sheep polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-Gdown1 Antibody (Sheep Polyclonal Antibody) validated in WB, ChIP to detect Gdown1 also known as Myocardial zonula adherens protein, GRINL1A upstream protein, Gup. |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Price, D., et al. (2012). Cell. 45(1):38-50.) Chromatin Immunoprecipitation Sequencing Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Price, D., et al. (2012). Cell. 45(1):38-50.) |
| Biological Information | |
|---|---|
| Immunogen | Histidine-tagged recombinant protein corresponding to human Gdown1. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Sheep |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~50 kDa observed. Uniprot describes 11 isoforms ranging between 44 and 64 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected Gdown1 on 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |