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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE2696 |
| Replaces | 07-1230 |
| Description | Anti-GADD45 alpha Antibody |
| Alternate Names |
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| Background Information | Growth arrest and DNA damage-inducible protein GADD45 alpha (UniProt P24522; also known as DDIT-1, DNA damage-inducible transcript 1 protein) is encoded by the GADD45A (also known as DDIT1, GADD45) gene (Gene ID 1647) in human. GADD45A, GADD45B, and GADD45G constitute a family of sensors that can mediate rapid responses to a variety of stressors in mammals. GADD45 proteins are involved in cellular DNA repair, cell cycle, cell survival, apoptosis, senescence, and DNA demethylation under various stress conditions. The interaction of GADD45 with PCNA regulates DNA replication and repair by stimulating nucleotide excision repair. GADD45 can induce cell cycle arrest through direct interaction with cell cycle-related protein CDK1 and p21. Direct phosphorylation of MEKK4 by GADD45 leads to activation of JNK and p38 kinase, resulting in cellular apoptosis and senescence, respectively. All GADD45 proteins are able to interact with each other and form homo- or hetero-dimers, which are crucial for GADD45 functions. GADD45 proteins are ubiquitously expressed, but can vary in their tissue expression patterns among the three members. Gadd45a deletion in mice causes genomic instability, resulting in defective mitosis and cytokinesis, as wll as increased radiation carcinogenesis. In addition to the reported antitumor activity via the induction of cellular apoptosis or senescence in cancer cells, GADD45A has also been shown to play a role in facilitating tumorigenesis in certain cancers. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-GADD45 alpha Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of GADD45 alpha. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected GADD45 alpha dimer in 10 µg of HEK293 cell lysate. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GADD45 alpha in human colon cancer tissue section. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the N-terminal sequence of human GADD45 alpha. |
| Epitope | N-terminus. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects GADD45 alpha. Immunogen sequence is not conserved in GADD45 beta. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Bovine, Porcine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~18/36 kDa (monomer/dimer) observed. 18.34 kDa (monomer) calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse kidney tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected GADD45 alpha in 10 µg of mouse kidney tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |