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Millipore Anti-T-Box Transcription Factor Tbx5 Antibody Detects Level Of T-Box Transcription Factor Tbx5 & Has Been Published & Validated For Use In Western Blotting, Icc & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
The gene encoding T-box transcription factor 5 (TBX5) is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity.
Product Information
Format
Serum
Control
HeLa cell lysate
Presentation
Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-T-box transcription factor TBX5 Antibody detects level of T-box transcription factor TBX5 & has been published & validated for use in Western Blotting, ICC & IHC.
Key Applications
Western Blotting
Immunocytochemistry
Immunohistochemistry
Application Notes
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected T-box transcription factor TBX5 in 293T cells overexpressing T-box transcription factor TBX5, mouse primary cardiocytes treated with serum, HA-tagged Tbx5 transfected NIH/3T3 cells, and HA-tagged Tbx5 transfected C2C12 cells (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.)
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected T-box transcription factor TBX5 in mouse embryonic tissue and in Tbx5 transgenic mouse heart tissues treated with tamoxifin (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.).
Biological Information
Immunogen
Recombinant protein corresponding to mouse T-box transcription factor TBX5.
Host
Rabbit
Species Reactivity
Human
Mouse
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_035667
Gene Symbol
Tbx5
Purification Method
Unpurified
UniProt Number
P70326
Molecular Weight
~53 kDa observed. An uncharacterized band may be observed at ~75 kDa in some cell lysates. Three isoforms due to alternative splicing may be observed at ~35 kDa, ~50 kDa, and ~64 kDa in some cell lysates. (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.).
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected T-box transcription factor TBX5 in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
