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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | DB, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE286 |
| Description | Anti-acetyl-p53 (Lys120) Antibody |
| Alternate Names |
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| Background Information | p53 is a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor that upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 polyubiquitinates p53 for proteasome targeting. p53 can assemble into tetramers in the absence of DNA, fluctuates between latent and active (DNA-binding) conformations, and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) (amino acids 110-286) of p53 can compromise energetically favorable association with cis elements and are implicated in several human cancers. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-acetyl-p53 (Lys120) Antibody is a highly specific rabbit polyclonal antibody, that targets p53 & has been tested in DOT Blot & IP. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-p53 in camptothecin treated HCT116 cell lysate. Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-p53 in p53 transfected H1299 human lung cotransfected with or without hMOF (Prof. S. McMahon, Kimmel Cancer Center of Thomas Jefferson Medical College). Immunoprecipitation Analysis: 1.5 µg or 4.5 µg from a representative lot immunoprecipitated endogenous acetyl-p53 in HCT116 cell lysates, which were treated with a vehicle (DMSO) or camptothecin (CPT) (Prof. S. McMahon, Kimmel Cancer Center of Thomas Jefferson Medical College). Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-p53 in H1299 cell lysates, which were transfected with either wild type p53, the 9 K/R mutant (all lysine residues except Lys120 mutated to arginine), the 10 K/R mutant (all lysine residues mutate including Lys 120), or empty vector, with or without hMOF (Prof. S. McMahon, Kimmel Cancer Center of Thomas Jefferson Medical College). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the AXIN1 interacting domain of human p53 acetylated at Lys120. |
| Epitope | AXIN1 interacting domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the AXIN1 interacting domain of p53 acetylated at Lys120. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with Sheep, Porcine, Rabbit, and Canine based on 100% sequence homology. Other homologies: Mouse, Rat, Bovine, Feline, Zebrafish, and Equine (88% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~43 kDa calculated. Many isoforms are known to exist. This antibody is predicted to react with isoform 1 (44 kDa), isoform 2 (38 kDa), isoform 3 (39 kDa), isoform 4 (39 kDa), isoform 5 (34 kDa), and isoform 6 (34 kDa). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by DOT Blot in non-acetylated and acetylated p53 peptides. DOT Blot Analysis: 1 µg/mL of this antibody detected acetyl-p53 in acetylated p53 peptides |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |