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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, DB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE328 |
| Description | Anti-dimethyl Histone H3 (Arg2) Antibody |
| Alternate Names |
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| Background Information | Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone H3 Arg2 methylation is a widespread silencing modification that inhibits histone H3 Lys4 trimethylation. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Histone H3 using this rabbit polyclonal antibody, Anti-dimethyl Histone H3 (Arg2) Antibody validated for use in western blotting & DOT Blot. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:620 dilution of this antibody detected dimethyl Histone H3 (Arg2) in CARM1 treated dimethyl Histone H3 (Arg2) recombinant protein, but demonstrated a loss of signal in untreated dimethyl Histone H3 (Arg2) recombinant protein. (Note: CARM1 is a methyl transferase that results in the methylation of Histone H3.). DOT Blot (Specificity) Analysis: A 1:620 dilution from a representative lot detected dimethyl Histone H3 (Arg2) peptides, but not unmethylated Histone H3 (Arg2) or other peptides corresponding to modified histones. Absurance™ is a trademark of EMD Millipore, Inc. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3 dimethylated at Arg2. |
| Epitope | N-terminus |
| Host | Rabbit |
| Specificity | This antibody recognizes the N-terminus of Histone H3 dimethylated at Arg2. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Broad species reactivity is expected. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa acid extract. Western Blotting Analysis: A 1:620 dilution from a representative lot detected dimethyl Histone H3 (Arg2) in 10 µg of HeLa acid extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |