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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE394 |
| Description | Anti-Cohesin subunit SA-2 Antibody |
| Alternate Names |
|
| Background Information | The cohesin subunit SA-2 is a part of the cohesin complex. This complex is comprised of a heterodimer between SMC1 and SMC3 proteins, as well as RAD21 and STAG proteins (STAG1, 2, or 3). These proteins form a large ring that can trap sister chromatids after DNA replication. During prophase, the cohesion complexes dissociate from the chromatin due to the phosphorylation by PLK1 (Polo-like kinase) with the exception at the centromeres where the cohesion complexes remain. Then during anaphase, the RAD21 subunit is cleaved which causes the dissociation of the complex from chromosomes, allowing chromosome separation. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Cohesin subunit SA-2 using this Anti-Cohesin subunit SA-2 Antibody validated for use in 0. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Cohesin Subunit SA-2 in A431 and HeLa cells. Immunoprecipitation Analysis: A representative lot immunoprecipitated Cohesin Subunit SA-2 from HeLa cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Cohesin Subunit SA-2. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with Rhesus Macaque, Equine, and Bovine based on 100% sequence homology. Other homologies: Mouse and Rat (93%-14/15aa). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~140 kDa observed. An uncharacterized band may appear at ~35 kDa in some lysates. Uncharacterized bands may appear at ~40 kDa and ~30 kDa in Immunoprecipitation. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell nuclear extract. Western Blot Analysis: 0.2 µg/mL of this antibody detected Cohesin Subunit SA-2 in 10 µg of HeLa cell nuclear extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |