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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, PIA | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE409 |
| Description | Anti-UTX Antibody |
| Alternate Names |
|
| Background Information | Lysine-specific demethylase 6A (UniProt: O15550; also known as EC:1.14.11.68, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein, [histone H3]-trimethyl-L-lysine(27) demethylase 6A, KDM6A, UTX) is encoded by the KDM6A (also known as UTX) gene (Gene ID: 7403) in human. UTX is a histone demethylase that specifically demethylates lysine 27 of histone H3 and plays a central role in histone code. It is shown to demethylate trimethylated and dimethylated, but not monomethylated H3 lysine 27. It mediates muscle regeneration through its demethylase activity, while it regulates mesoderm differentiation of embryonic stem cells and mammary gland development independent of its demethylase activity. It also plays a role in regulating brown adipocyte thermogenic program. Its levels are shown to be up-regulated during brown adipocyte differentiation and by cold exposure in both brown and white adipose tissue in mice, indicating its potential role in thermogenesis. Mutations in KDM6A gene are a causative factor in Kabuki syndrome that is characterized by congenital mental retardation, postnatal dwarfism, large prominent earlobes, a cleft or high-arched palate, scoliosis, and other abnormalities. (Ref.: Zha, L et Al. (2015). J. Biol. Chem. 290, 25151-25163; Lederer, D et Al. (2012). Am. J. Hum. Genet. 90, 119-124). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-UTX, Cat. No. ABE409, is a rabbit polyclonal antibody that detects Histone demethylase UTX and is tested for use in Immunocytochemistry, Peptide Inhibition Assay, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected UTX in HeLa, A431, and NIH3T3 cells. Peptide Inhibition Assay: Target band detection in nuclear extract of HeLa cells was prevented by preblocking of a representative lot with the recombinant UTX protein. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to 82 amino acids from the N-terminal half of human Histone demethylase UTX. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Histone demethylase UTX. It targets an epitope within the N-terminal half. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Bovine, Canine, Chimpanzee based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Antigen Affinity Purified |
| UniProt Number |
|
| Molecular Weight | ~155 kDa observed; 154.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in nuclear extract of HeLa cells. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UTX in nuclear extract of HeLa cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |