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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, ChIP, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE425 |
| Description | Anti-Jarid2 Antibody |
| Alternate Names |
|
| Background Information | Protein Jumonji (Jarid2), also known as Jumonji/ARID domain-containing protein 2, and encoded by the gene name Jarid2 is a member of the JARID2 family. Jarid 2 is a regulator of histone methyltransferase complexes, and therefore plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Recent studies have shown that Jarid 2 modulates histone methyltransferase activity and promotes the recruitment of histone methyltransferase complexes to their target genes. However, Jarid 2 does not have histone demethylase activity. Jarid 2 binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. In embryonic stem cells, Jarid 2 associates with the PRC2 complex and inhibits trimethylation of Lys-27 of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, Jarid 2 is required to repress expression of cyclin-D1 (CCND1) by activating methylation of Lys-9 of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Jarid 2 has been implied to act as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. In addition, Jarid 2 might participate in the negative regulation of cell proliferation signaling. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal serum in buffer containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Jarid2 is an antibody against Jarid2 for use in WB, ChIP;IH. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot of this antibody detected Jarid2 in mouse embryo extracts at E12.5. Immunoprecipitation Analysis: A representative lot of this antibody immunopreciptated Jarid2 from HEK293 transfected cell lysates (Mysliwiec, M.R., et al. (2007) FEBS. 581(14):2633-2640). Chromatin Immunoprecipitation Analysis (ChIP-chip): A representative lot of this antibody immunopreciptated Jarid2 using chromatin from E17.5 Mouse heart tissue lysate using ChIP-chip (Mysliwiec, M.R., et al. (2011) JBC. 287(2):1235-1241). Immunohistochemistry Analysis: A representative lot of this antibody detected Jarid2 in E17.5 Mouse heart tissue sections (Mysliwiec, M.R., et al. (2011) JBC. 287(2):1235-1241). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to mouse Jarid2. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~57 kDa observed from recombinant protein. Endogenous Jarid2 protein has a calculated molecular weight of 137 kDa. Uncharacterized bands may be observed in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting using recombinant protein GST-Jarid2 (aa529-798). Western Blotting Analysis: A 1:1,000 dilution of this antibody detected 0.2 µg of recombinant protein GST-Jarid2 (aa529-798). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |