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Millipore This Anti-Brdt Antibody Is Validated For Use In Immunohistochemistry For The Detection Of Brdt. - Mill (Additional S&H or Hazmat Fees May Apply)
Bromodomain-containing female sterile homeotic-like protein
RING3-like protein
Background Information
Bromodomain testis-specific protein (BRDT), also known as Bromodomain-containing female sterile homeotic-like protein, or RING3-like protein, and encoded by the gene BRDT/ FSRG3 is a testis-specific chromatin protein that binds specifically histone H4 acetylated at Lys-5 and Lys-8 (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Recent studies have shown that BRDT plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. Therefore, it is involved in the late pachytene spermatocytes development. In the post-meiotic phase of spermatogenesis, BRDT binds to hyperacetylated histones and participates in their general removal from DNA. In addition, BRDT acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids, and participates in 3-UTR truncation of specific mRNAs in post-meiotic spermatids. BRDT is required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin. BRDT is testis-specific, and expressed in the germinal cells: in the early meiotic (pachytene) spermatocytes during spermiogenesis, in the round and elongating spermatids , and in the condensed late spermatids.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-BRDT antibody is validated for use in Immunohistochemistry for the detection of BRDT.
Key Applications
Immunohistochemistry
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to mouse BRDT.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_473395
Gene Symbol
Brdt
Fsrg3
Purification Method
Affinity Purfied
UniProt Number
Q91Y44
Molecular Weight
107 kDa calculated
Product Usage Statements
Quality Assurance
Evaluated by Immunohistochemistry in mouse testis tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected BRDT in mouse testis tissue.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
