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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE438 |
| Description | Anti-Apobec1 Antibody |
| Alternate Names |
|
| Background Information | C->U-editing enzyme APOBEC-1 (UniProt: P41238; also known as EC:3.5.4.36, Apolipoprotein B mRNA-editing enzyme 1, HEPR, mRNA(cytosine(6666)) deaminase 1) is encoded by the APOBEC1 gene (Gene ID: 339) in human. Apobec1 is a homodimeric protein that is expressed exclusively in mammalian intestines. It is the catalytic component of the apolipoprotein B mRNA editing enzyme complex and serves as a zinc-dependent cytidine deaminase that converts a specific cytidine to uridine in apolipoprotein B (ApoB) mRNA transcripts. Hence, it is responsible for the post-transcriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Its CMP/dCMP-type deaminase domain is localized in amino acids 10-134. Overexpression of Apobec1 has been linked to hepatocellular carcinoma in mice and rabbits and its deficiency in cancer-prone mice is reported to reduce the number of polyps and tumors in the gastrointestinal tract. (Ref.: Saraconi, G., et al. (2014). Genome Biol. 15; Article number 417; Rosenberg, BR., et al. (2011). Nat. Struct. Biol. 18(2); 230-236). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Apobec1, Cat. No. ABE438, is a rabbit polyclonal antibody that detects Apobec1 and is tested for use in Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Tested Applications Immunohistochemistry (Paraffin) Analysis: A 1:500-2,000 from a representative lot detected Apobec1 in Rat Small Intestine and Human colon tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 14 amino acids from the internal region of human C->U-editing enzyme Apobec1. |
| Epitope | Internal |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects C->U-editing enzyme Apobec1. It targets an epitope within 14 amino acids from the internal region. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~30 kDa observed; 28.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Rat Spleen tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Apobec1 in Rat spleen tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |