Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE440 |
| Description | Anti-RAG-1 Antibody |
| Alternate Names |
|
| Background Information | V(D)J recombination-activating protein 1 (RAG-1) is also called RING finger protein 74. RAG-1 is a component of the RAG complex, which mediates the DNA cleavage phase during V(D)J recombination. As part of the RAG complex, RAG1 mediates the DNA-binding and catalyzes the DNA cleavage activities by introducing a double-strand breaks between the recombination signal sequences (RSS) and the adjacent coding segment. RAG-1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3 as well as polyubiquitination of KPNA1. RAG-1 is expressed in maturing lymphoid cells. RAG-1 defects can cause disease states such as: combined cellular and humoral immune defects with granulomas (CHIDG), severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive (T-B-NK+ SCID), Omenn syndrome (OS), and alpha/beta T-cell lymphopenia with gamma/delta T-cell expansion severe cytomegalovirus infection and autoimmunity (T-CMVA). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect RAG-1 using this rabbit polyclonal antibody, Anti-RAG-1 Antibody validated for use in western blotting, ICC & IP. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1 µg/mL from a representative lot detected RAG-1 immunopreciptated from 500 µg of HL-60 cell lysate. (Note: It is recommended to immunoprecipitate RAG-1 before performing WB). Immunocytochemistry Analysis: 1:100 dilution from a representative lot detected RAG-1 in Jurkat cells. DyLight® is a registered trademark of Thermo Fisher Scientific. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a region near the N-terminus of human RAG-1. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~120 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunoprecipitation in HL-60 cell lysate. Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated RAG-1 in 500 µg of HL-60 cell lysate. Immunopreciptated protein was then probed with 1 µg/mL of the same antibody in WB. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |