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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IH(P), PIA | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE457 |
| Description | Anti-c-Fos Antibody |
| Alternate Names |
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| Background Information | Proto-oncogene c-Fos (UniProt: P01100; also known as Cellular oncogene fos, G0/G1 switch regulatory protein 7) is encoded by the FOS (also known as G0S7) gene (Gene ID: 2353) in human. C-Fos is a nuclear phosphoprotein that forms a tight, but non-covalently linked, complex with the JUN/AP-1 transcription factor. In the heterodimer, c-Fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. In quiescent cells, the small amount of c-Fos present is phosphorylated at tyrosine 10 and 30. This tyrosine phosphorylated form is cytosolic. Following induction of cell growth, it first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum is shown to require dephosphorylation at tyrosine 10 and 30. In growing cells, c-Fos is dephosphorylated by PTPN2, which leads to its association with endoplasmic reticulum membranes and activation of phospholipid synthesis. A basic motif (aa 139-159) is required for the activation of phospholipid synthesis. Upon stimulation by nerve growth factor it is undergoes phosphorylation in the C-terminal region by MAPK and RSK1. Phosphorylation on both serine 362 and serine 374 leads to its stabilization. Phosphorylation on serine 362 and serine 374 primes further phosphorylation on threonine 325 and 331 through promoting docking of MAPK to the DEF domain. c-Fos is also reported to be constitutively sumoylated with SUMO1, SUMO2 and SUMO3 and sumoylation requires heterodimerization with JUN and is enhanced by mitogenic stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on threonine 232. Three different isoforms of c-Fos have been described that are produced by alternative splicing. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-c-Fos, Cat. No. ABE457, is a rabbit polyclonal antibody that detects Proto-oncogene c-Fos and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected c-Fos in rat brain tissue sections. Peptide Inhibition Assay: Target band detection in lysate from HeLa cells stimulated with TPA (16 nM for 8 h) was prevented by pre-blocking of a representative lot with the immunogen peptide. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human c-Fos. |
| Epitope | N-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects c-Fos in multiple species. It targets an epitope with in 14 amino acids from the N-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat. Predicted to react with Bovine, Chimpanzee, Rhesus Monkey, Porcine, Sheep based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | 56 kDa observed. 40.7 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cells stimulated with TPA. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected c-Fos in lysate from Phorbol myristate acetate (PMA) treated (16 nM; 8 hours) HeLa cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |