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Millipore Use Anti-Gal4 (Activation Domain) Antibody (Rabbit Polyclonal Antibody) Validated In Wb To Detect Gal4 (Activation Domain) Also Known As Regulatory Protein Gal4. - Mill (Additional S&H or Hazmat Fees May Apply)
GAL4 is a transcriptional activator which controls the expression of genes involved in the catabolism of galactose. In addition to a DNA-binding domain, Gal4 contains two acidic regions: activating region I (aa148-238) and activating region II (aa768-881), which can both activate transcription when independently joined to the DNA-binding domain. The activation domains of Gal4 have been shown to interact with Gal80, TBP, SUG1, and ADA2, among other general transcription factors.
Product Information
Format
Affinity Purified
Control
Gal4 positive and Gal4 negative yeast
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Use Anti-GAL4 (activation domain) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect GAL4 (activation domain) also known as Regulatory protein GAL4.
Key Applications
Western Blotting
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the C-terminus of yeast GAL4 (activation domain).
Epitope
C-terminus
Host
Rabbit
Specificity
This antibody recognizes the C-terminus of GAL4 (activation domain).
Species Reactivity
Yeast
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_015076NP_015076NP_015076NP_015076vdNP_015076
Gene Symbol
GAL4
Purification Method
Affinity Purfied
UniProt Number
P04386
Molecular Weight
~28 kDa, ~38 kDa, ~77 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in Gal4 positive and Gal4 negative yeast cell lysate.
Western Blot Analysis: A 1:160,000 dilution of this antibody detected Gal4 in 5 mg of Gal4 positive (+ recombinant Gal4) yeast cell lysate. This antibody also detected small mounts of endogenous Gal4 in Gal4 negative (- recombinant Gal4) yeast cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
