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Millipore Anti-Phospho-Stat2 (Tyr689) Antibody Is An Antibody Against Phospho-Stat2 (Tyr689) For Use In Immunoprecipitation. - Mill (Additional S&H or Hazmat Fees May Apply)
The Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signaling pathway plays an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. Constitutive activation or dysregulation of JAK/STAT signaling can result in inflammatory disease, erythrocytosis, gigantism, and leukemia. STAT2 (113 kDa), originally purified from the nuclei of a-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta. Knockout mice with a targeted disruption of STAT2 exhibit greater susceptibility to viral infection and altered responses to Type I interferons. Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via assocations with receptor-bound Jak kinases.
Product Information
Format
Affinity Purified
Control
Untreated and IFN alpha/gamma treated HeLa cell lysate.
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-phospho-STAT2 (Tyr689) Antibody is an antibody against phospho-STAT2 (Tyr689) for use in Immunoprecipitation.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho-STAT2 (Tyr689) from IFN alpha treated HeLa cell lysate.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to mouse STAT2 phosphorylated at Tyr689.
Epitope
Phosphorylated Tyr689.
Host
Rabbit
Specificity
This antibody recognizes STAT2 phosphorylated at Tyr689.
Species Reactivity
Human
Mouse
Species Reactivity Note
Demonstrated to react with Human. Predicted to react with Mouse based on 100% sequence homology. Other homologies: Rat (89% sequence homology).
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_064347
Gene Symbol
STAT2
Purification Method
Affinity Purfied
UniProt Number
Q9WVL2
Molecular Weight
~113 kDa observed. An uncharacterized band may appear at ~260 kDa in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:200 dilution of this antibody detected phospho-STAT2 (Tyr689) in 10 µg of IFN alpha/gamma treated HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
