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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE542 |
| Description | Anti-YTHDF2 Antibody |
| Alternate Names |
|
| Background Information | YTH domain-containing family protein 2 (YTHDF2), also known as CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2, is also encoded by the gene name YTHDF2 and HGRG8. YTHDF2 recognizes specifically and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs. Recent studies have shown that YTHDF2 may play an important role in the efficiency of mRNA splicing, processing and stability. Additionally, YTHDF2 has been shown to act as a regulator of mRNA stability. And in the process of binding to m6A-containing mRNAs, YTHDF2 has been implicated to localize to mRNA decay sites, such as processing bodies (P-bodies), which leads to mRNA degradation. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-YTHDF2 antibody is validated for use in WB, IC for the detection of YTHDF2. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 15 µg/mL from a representative lot detected YTHDF2 in WT HeLa cell lysate and YTHDF2 overexpressing lysate (Chuan He, Univerity of Chicago). Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected YTHDF2 in HeLa cells (Chuan He, Univerity of Chicago). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the localization of mRNA processing bodies (P-bodies) to human YTHDF2. |
| Epitope | Localization to mRNA processing bodies (P-bodies) |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~62 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in C6 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected YTHDF2 in 10 µg of C6 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |