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Millipore Detect Ku70 Protein Using This Rabbit Polyclonal Antibody, Anti-Ku70 Protein Antibody Validated For Use In Western Blotting, Ip & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
X-ray repair complementing defective repair in Chinese hamster cells 6
Background Information
Lupus Ku autoantigen protein p70 (Ku70) is also known as X-ray repair cross-complementing protein 6 (XRCC6), 5-dRP lyase Ku70, CTC box-binding factor 75 kDa subunit (CTC75, CTCBF) or Thyroid-lupus autoantigen (TLAA). Ku70 is a single stranded DNA-dependent ATP-dependent helicase that plays a role in chromosome translocation and DNA non-homologous end joining (NHEJ) required for double-strand break repair and recombination. The XRCC5/6 (Ku80/Ku70) dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK, and that dimer, together with APEX1, acts as a negative regulator of transcription. Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to XRCC5 (Ku80) and XRCC6 (Ku70).
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Ku70 Protein using this rabbit polyclonal antibody, Anti-Ku70 Protein Antibody validated for use in western blotting, IP & IHC (Paraffin).
Key Applications
Western Blotting
Immunoprecipitation
Immunohistochemistry (Paraffin)
Application Notes
Immunoprecipitation Analysis: A representative lot immunoprecipitated Ku70 Protein in 0.5 mg of HeLa cell lysate. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Ku70 Protein in human prostate carcinoma cells and human testis tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human Ku70 Protein near N-Terminus.
Epitope
Near N-Terminus
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes Ku70 Protein near N-Terminus.
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001460
Gene Symbol
XRCC6
G22P1
Purification Method
Protein A Purfied
UniProt Number
P12956
Molecular Weight
~70 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ku70 Protein in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
