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Millipore Detect Special At-Rich Sequence-Binding Protein 1 Using This Rabbit Polyclonal Antibody, Anti-Satb1 Antibody Validated For Use In Western Blotting, Ihc (Paraffin) & Immunofluorescence. - Mill (Additional S&H or Hazmat Fees May Apply)
Special AT-rich sequence-binding protein 1 (SATB1) contains 2 CUT DNA-binding domains and one homeobox DNA binding domain belonging to the CUT homeobox family. SATB1 contributes to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. SATB1 is organized into a cage-like network anchoring loops of heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex), and transcription factors recruitment. SATB1 is predominantly expressed in thymus. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic.
Product Information
Format
Affinity Purified
Control
A20 cell lysate
Presentation
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Special AT-rich sequence-binding protein 1 using this rabbit polyclonal antibody, Anti-SATB1 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Immunofluorescence
Application Notes
Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected SATB1 in human brain tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected SATB1 in human brain cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human SATB1.
Epitope
C-terminus
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the C-terminus of SATB1.
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Gene Symbol
SATB1
Purification Method
Antigen Affinity Purified
UniProt Number
Q01826
Molecular Weight
~90 and ~98 kDa observed. Uniprot describes two isoforms at ~86 kDa and ~89 kDa. Uncharacterized bands may appear in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in A20 cell lysate.
Western Blotting Analysis: 4 µg/mL of this antibody detected SATB1 in 15 µg of A20 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
