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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC, WB, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE68 |
| Description | Anti-FoxP1 Antibody |
| Alternate Names |
|
| Background Information | Forkhead Box P1 (FoxP1) is a member of the FOX family of transcription factors. This protein is known to forms homodimers and heterodimers with family members FoxP2 and FoxP4. The forkhead box family has a broad range of functions. These proteins play important roles in immune response, organ development and the development of cancer. The FoxP1 protein is widely expressed and contains both forkhead (winged-helix) and C2H2 zinc finger nucleic acid-binding motifs. At least four different isoforms that result from alternative splicing of the mRNA transcript have been reported. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Unpurified rabbit polyclonal serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-FoxP1 Antibody (Rabbit Polyclonal Antibody) validated in ICC, WB, IHC to detect FoxP1 also known as forkhead box P1, fork head-related protein like B, glutamine-rich factor 1. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected FoxP1 in HeLa and NIH/3T3 cells. Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Lu, M.M., et al. (2002). Mechanisms of Development 119S:S197–S202.) |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to mouse FoxP1. |
| Epitope | Unknown |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes FoxP1. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq] |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Transcriptional repressor that plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. SUBUNIT STRUCTURE: Forms homodimers and heterodimers with FOXP2 and FOXP4. Dimerization is required for DNA-binding. Interacts with CTBP1. SUBCELLULAR LOCATION: Nucleus Probable. TISSUE SPECIFICTY: Highest expression in the lung, brain, and spleen. Lower expression in heart, skeletal muscle, kidney, small intestine (isoform 3 not present) and liver. DEVELOPMENTAL STAGE: Expressed in developing lung, neural, intestinal and cardiovascular tissues. Expressed in both the airway epithelium of the forming lung as well as in the surrounding mesenchyme. By 16.5 dpc, expressed throughout the conducting airway epithelium, with highest expression in the distal alveolar regions. Also expressed in the endotheial cells of the pulmonary vasculature. During intestinal development, expressed in the mucosal layer but absent from the epithelium at 12.5 dpc. By 16.5 dpc, expressed in both the inner circular and outer longitudinal muscular layers of the intestine as well as in the epithelium of the intestine and developing stomach. DOMAIN: The leucine-zipper is required for dimerization and transcriptional repression. SEQUENCE SIMILARITIES: Contains 1 C2H2-type zinc finger. Contains 1 fork-head DNA-binding domain. |
| Molecular Weight | 79 kDa observed. Alternative splicing can result in cell type dependent isoforms. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in RAW264.7 cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected FoxP1 on 10 µg of RAW264.7 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |