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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, Rb, H | WB, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE73 |
| Description | Anti-FoxP2 Antibody |
| Alternate Names |
|
| Background Information | Forkhead box protein P2 (FoxP2) is a multidomain transcriptional repressor that belongs to the Fox family of proteins. The FoxP2 protein contains a forkhead-box DNA-binding domain, characteristic of the FOX group of transcription factors. In addition, this protein contains a polyglutamine tract, as well as zinc finger and leucine zipper motifs. In order for FoxP2 to bind DNA, it forms homodimers and heterodimers with FoxP1 and FoxP4. The forkhead box family of proteins play an important role in immune response, organ development and the development of cancer. Functional studies in vertebrates suggest this with this gene plays a role in the modulation neural plasticity. In line with these findings, mutations of FoxP2 in humans have been linked to speech and language disorders. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Unpurified rabbit polyclonal serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-FoxP2 Antibody is a Rabbit Polyclonal Antibody for detection of FoxP2 also known as forkhead box P2, trinucleotide repeat containing 10 & has been validated in WB & IHC. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 1:1,000 dilution from a representative lot detected FoxP2 in 10 µg of L6, NIH/3T3, mouse brain, rat brain, and human brain lysates. Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Lu, M.M., et al. (2002). Mechanisms of Development 119S:S197–S202.) |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to mouse FoxP2. |
| Epitope | Unknown |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes FoxP2. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human, mouse, and rabbit. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. SUBUNIT STRUCTURE: Forms homodimers and heterodimers with FOXP1 and FOXP4. Dimerization is required for DNA-binding. Interacts with CTBP1. SUBCELLULAR LOCATION: Nucleus Probable. TISSUE SPECIFICITY: Highest expression in lung. Lower expression in spleen, skeletal muscle, brain, kidney and small intestine. DEVELOPMENTAL STAGE: Expressed in developing lung, neural, intestinal and cardiovascular tissues. Expressed at a high level in the distal airway epithelium and at a low level in the proximal airway epithelium at 12.5 dpc, and restricted to the distal airway epithelium by 14.5 dpc. In the spinal cord, at 12.5 dpc, expressed in a subset of interneurons dorsal to motor neurons. At 16.5 dpc, expression in the brain is observed in the inner intermediate zone of the neopallial cortex and in the developing cerebral hemispheres. In the gastrointestinal system, at 12.5 expressed in the outer mesodermal layer and in the intestinal epithelium. By 16.5 dpc, expression is restricted to the outer longitudinal muscle layer of the intestine and stomach. In the cardiovascular system, at 14.5 dpc, expressed in the outflow tract region of the developing heart. By 16.5 dpc, observed in the outflow tract and atrium, but not in the ventricles. DOMAIN: The leucine-zipper is required for dimerization and transcriptional repression. SEQUENCE SIMILARITIES: Contains 1 C2H2-type zinc finger. Contains 1 fork-head DNA-binding domain. |
| Molecular Weight | 80 and 77 kDa Observed. Alternative splicing can result in cell type dependent isoforms. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in RAW264.7 cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected FoxP2 on 10 µg of RAW264.7 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |