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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC, WB, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE74 |
| Description | Anti-FoxP4 Antibody |
| Alternate Names |
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| Background Information | Forkhead box protein P4 (FoxP4) is a multidomain transcriptional repressor. This protein is a member of the Fox family of proteins characterized by it unique winged-helix DNA binding motif. Members of this family are known for their ability to regulate both the cell fate and differentiation of several tissues. FoxP4 acts to repress expression by inteacting with DNA. For DNA binding to occur, FoxP4 must form homodimers and heterodimers with FoxP1 and FoxP2. During embryogenesis, expression of this protein is observed in lung and brain. In adults expression is observed in adult heart, lung, brain, liver, spleen, testis and kidney. Alternative splicing result in the expression of three known isoforms. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Unpurified rabbit polyclonal serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-FoxP4 Antibody (Rabbit Polyclonal Antibody) validated in ICC, WB, IHC to detect FoxP4 also known as forkhead box P4, winged-helix repressor FOXP4. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected FoxP4 in HeLa cells. Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Lu, M.M., et al. (2002). Mechanisms of Development 119S:S197–S202.) |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to mouse FoxP4. |
| Epitope | Unknown |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes FoxP4. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Transcriptional repressor that represses lung-specific expression. SUBUNIT STRUCTURE: Forms homodimers and heterodimers with FOXP1 and FOXP2. Dimerization is required for DNA-binding. SUBCELLULAR LOCATION: Nucleus Probable. TISSUE SPECIFICITY: Expressed in the adult heart, brain, spleen lung, liver, kidney and testes. DEVELOPMENTAL STAGE: Expressed predominantly in the lung and brain during embryogenesis. Expressed in the lung epithelium and the mesenchyme immediately adjacent to the epithelium from 10.5 dpc. At 9.5 dpc, expressed in the foregut endoderm but not in the heart. At 16.5 and 18.5 dpc, expressed in both the proximal and distal airway epithelium. Also expressed in the developing gut. In the hindgut, primarily expressed in epithelial cells of the intestine and stomach. Expressed in the brain in a dynamic pattern. At 14.5 dpc, expressed at high levels in the intermediate zone of the neopallial cortex with lower levels in the surrounding cells. By 16.5 dpc, no longer expressed in the intermediate zone, but still present in the surrounding cells of the neopallial cortex. DOMAIN: The leucine-zipper is required for dimerization and transcriptional repression. SEQUENCE SIMILARITIES: Contains 1 C2H2-type zinc finger. Contains 1 fork-head DNA-binding domain. |
| Molecular Weight | 86 and 79 kDa Observed. Three known isoforms due to alternative splicing. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human brain cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected FoxP4 on 10 µg of human brain cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |