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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE75 |
| Description | Anti-FOXP3 Antibody |
| Alternate Names |
|
| Background Information | FOXP3 (forkhead box P3) is a member of the FOX protein family and is thought to function as the master regulator in the development and function of regulatory T cells. As a member of the forkhead/winged-helix family of transcriptional regulators FOXP3 is believed to regulate expression through DNA binding. Mutations and deletions in the sequence encoding for FOXP3 expression lead to an inability to express FOXP3, causing an inability to regulate T cell development and leading to fatal autoimmune diseases such as IPEX and XLAAD. Conversely, other studies have shown high and wide expression of FOXP3 in several cancer cell lines and tumors, indicating that FOXP3 is involved in preventing immunity against malignant tumors. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit polyclonal serum containing 0.5% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-FOXP3 Antibody (Rabbit polyclonal antibody) validated in WB, ICC to detect FOXP3 also known as forkhead box P3. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected FOXP3 in A431 cells. |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to human FOXP3. |
| Host | Rabbit |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Probable transcription factor. Plays a critical role in the control of immune response. SUBCELLULAR LOCATION: Nucleus (Potential). INVOLVEMENT IN DISEASE: Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. SEQUENCE SIMILARITIES: Contains 1 C2H2-type zinc finger. Contains 1 fork-head DNA-binding domain. |
| Molecular Weight | ~50 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HEK293 cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected FOXP3 on 10 µg of HEK293 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |