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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1367 |
| Description | Anti-Pan DIDO Antibody |
| Alternate Names |
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| Background Information | Death-inducer obliterator 1 (UniProt Q9BTC0; also known as DATF-1, Death-associated transcription factor 1, DIO-1, hDido1) is encoded by the DIDO1 (also known as C20orf158, DATF1, KIAA0333) gene (Gene ID 11083) in human. The DIDO1 gene encodes four alternatively spliced variants (DIDO1/isoform 2, DIDO2/isoform 1, DIDO3/isoform 4, isoform a/isoform 3) implicated to be involved in apoptosis and development. The longest and most widely expressed DIDO3 is assigned as the canonical isoform. DIDO3 is a nuclear protein that forms part of the spindle assembly checkpoint (SAC) and is necessary for correct chromosome segregation in somatic and germ cells. Dido3-knockout compromises differentiation of murine embryonic stem cells (mESCs) in vitro and of epiblast cells in vivo, resulting in early embryonic death at around day 8.5 of gestation. On the other hand, exogenous expression of Dido1 isoform in mESCs is reported to inhibit LIF withdrawal-induced differentiation, while Dido1 knockdown facilitate mESCs differentiation. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Pan DIDO Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of DIDO. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected DIDO immunoreactivity in human kindey, human prostate cancer and human cerebellum tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding an N-terminal sequence of human DIDO1. |
| Epitope | N-terminal region. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects an N-terminal epitope present in all four spliced isoforms of human DIDO1 reported by UniProt (Q9BTC0). |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Mouse, Xenopus, Non-Human Primate based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~260/130/79/70/66 observed. 59.46 kDa (isoform 2; DIDO1), 129.2 kDa (isoform 1; DIDO2), 243.9 kDa (isoform 4; DIDO3), 61.35 kDa (isoform 3; isoform a) calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected DIDO isoforms in 10 µg of HepG2 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |