Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IP, IHC, IF | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN187 |
| Description | Anti-LRRK2 Antibody |
| Alternate Names |
|
| Background Information | LRRK2 (Leucine-rich repeat serine/threonine-protein kinase 2) is a multifunctional protein belonging to the ROCO family of proteins. LRRK2 contains multiple domains including a ROC (Ras of Complex protein) domain that binds and hydrolyzes GTP, and a kinase domain involved in the phosphorylation of myelin basic protein (MBP), mosein, ezrin, and radixin. LRRK2 itself undergoes autophosphorylation and is targeted by other kinases that modify multiple serine residues at its N-terminus; It is constitutively phosphorylated at Ser935 and maintains this state by interaction with the 14-3-3 protein. Although the kinases upstream of LRRK2 have not been definitively identified, previous studies have suggested that this group of kinases may include PKA. It also contains multiple leucine-rich repeat motifs that facilitate interaction with a range of proteins including PARK2 and PRDX3. Mutations in LRRK2, most of which have enhanced its kinase activity, may contribute to the development of late-onset familial Parkinson’s disease; however, the precise molecular mechanism of LRRK2-induced Parkinson’s disease is still unclear. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-LRRK2 Antibody is validated for use in WB, IH(P) for the detection of LRRK2. This antibody is known as JH5514 in scientific publications. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: A representative lot was used by an independent laboratory in HEK-293T cells transiently transfected with myc-tagged WT or mutant LRRK2. (West, A., et Al. (2005). PNAS. 102(46):16842–16847.) Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in neurons from mouse forebrain tissue. (Higashi, S., et Al. (2007). Journal of Neurochemistry. 100:368–381.) Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in transfected HEK-293T cells. (West, A., et Al. (2005). PNAS. 102(46):16842–16847.) Immunofluorescence Analysis: A representative lot was used by an independent laboratory in transfected HEK-293T cells. (West, A., et Al. (2005). PNAS. 102(46):16842–16847.) |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human LRRK2. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~290 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse brain tissue lysate. Western Blot Analysis: 1 µg/mL of this antibody detected LRRK2 in 10 µg of mouse brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |