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Millipore Detect Prohibitin Using This Rabbit Polyclonal Antibody, Anti-Prohibitin Antibody Validated For Use In Western Blotting & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Prohibitin is an inhibitor of DNA synthesis that is widely expressed across various tissues. Prohibitin influences proliferation, and may also have a role in regulating mitochondrial respiration activity and in aging. Expression levels of prohibitin in fibroblasts have been found to decrease heterogeneously as cellular aging occurs.
Product Information
Format
Affinity Purified
Control
Human liver tissue lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Prohibitin using this rabbit polyclonal antibody, Anti-Prohibitin Antibody validated for use in western blotting & IHC (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:100-500 dilution from a representative lot detected Prohibitin in human kidney and human ileum tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human Prohibitin near the N-Terminus.
Epitope
Near N-Terminus
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes Prohibitin near the N-Terminus.
Species Reactivity
Human
Species Reactivity Note
Demonstrated to react with Human. Predicted to react with Rat, Bovine, and Canine based on 100% sequence homology. Other homologies: Mouse (86% sequence homology).
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_002625
Gene Symbol
PHB
Purification Method
Affinity Purfied
UniProt Number
P35232
Molecular Weight
~32 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human liver tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Prohibitin in 10 µg of human liver tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
