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Apolipoprotein D (ADOP), also known as Apolipoprotein D, Apo-D, ApoD, and encoded by the gene name APOD, is a member of the calycin superfamily and the Lipocalin family. Recent studies have suggested that Apolipoprotein D is involved in the transport and binding of bilin. Additioanlly, studies have demonstrated that Apolipoprotein D is able to transport a variety of ligands in a number of different contexts. Apolipoprotein D is a homodimer located in the plasma, and it also exists as a disulfide-linked heterodimer with APOA2. Apolipoprotein D is expressed in the liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears. Apolipoprotein D is primarily localized in HDL (60-65%), with most of the remainder in VHDL and only small amounts in VLDL and LDL.
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Apolipoprotein D is an antibody against Apolipoprotein D for use in ELISA, WB.
Key Applications
ELISA
Western Blotting
Application Notes
ELISA Analysis: A representative lot of this antibody detected endogenous Apolipoprotein D in normal human plasma (Knipping, G., et al. Journal of Immunological Methods 202 1997. 85–95) Western Blotting Analysis: A representative lot of this antibody at 1:5000 dilution detected endogenouse Apolipoprotein D in 5 µl of human plasma (Bottcher, A., et al. J. Lipid Res. 2000. 41: 905–915.)
Biological Information
Immunogen
Protein purified from human plasma corresponding to Apolipoprotein D.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001638
Gene Symbol
APOD
Purification Method
Unpurified
UniProt Number
P05090
Molecular Weight
~30 kDa observed, additional bands likely due to heavy N-glycosylation on this protein
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting on purified human Apolipoprotein D
Western Blotting Analysis: A 1:1000 dilution of this antibody detected 0.05 µg of purified human Apolipoprotein D
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
"Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
