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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1848 |
| Description | Anti-Rho (-A, -B, -C) Antibody |
| Alternate Names |
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| Background Information | Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Rho (-A, -B, -C) Antibody is an antibody against Rho (-A, -B, -C) for use in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated synthetic peptide (SYPDTDVILMCFSIDSPDSLEN-KK) corresponding to amino acids 73-94 of human RhoA & RhoB, with two C-terminal lysines added to increase peptide solubility corresponding to human Rho (-A, -B, -C). |
| Epitope | Amino acids 73-94. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody is raised against an internal sequence highly conserved among RhoA (100%), RhoB (95%), and RhoC (100%). |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Rat, Chicken, Mouse, Nematode, Zebrafish, Xenopus based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~22 kDa observed. 21.44 kDa (human RhoA), 21.78 kDa (human RhoB), and 21.68 kDa (human RhoC) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HL-60 cell lysate. Western Blotting Analysis: A 1:5,000 dilution of this antibody detected RhoA family GTPase in 10 µg of HL-60 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |