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Millipore Anti-Rho (-A, -B, -C) Antibody Is An Antibody Against Rho (-A, -B, -C) For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2).
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Rho (-A, -B, -C) Antibody is an antibody against Rho (-A, -B, -C) for use in Western Blotting.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated synthetic peptide (SYPDTDVILMCFSIDSPDSLEN-KK) corresponding to amino acids 73-94 of human RhoA & RhoB, with two C-terminal lysines added to increase peptide solubility corresponding to human Rho (-A, -B, -C).
Epitope
Amino acids 73-94.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This polyclonal antibody is raised against an internal sequence highly conserved among RhoA (100%), RhoB (95%), and RhoC (100%).
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Rat, Chicken, Mouse, Nematode, Zebrafish, Xenopus based on 100% sequence homology.
Evaluated by Western Blotting in HL-60 cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected RhoA family GTPase in 10 µg of HL-60 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
