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Millipore Anti-Phospho Nfkb P65 Antibody, (Ser547) Is An Antibody Against Phospho Nfkb P65 Antibody For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Background Information
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa B forms multiple homo and heterodimeric complexes, one of which is with RELA/p65. The NF-kappa-B p65 complex appears to be involved in invasin-mediated activation of IL-8 expression, as well as the inhibitor effect on I-kappa-B. Phosphorylation of p65 at serine 547 by the nuclear kinase ATM appears to repress NF-κB-dependent transcription of specific genes induced by genotoxic stress.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-phospho NFkB p65 Antibody, (Ser547) is an antibody against phospho NFkB p65 Antibody for use in Western Blotting.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human phospho NFkB p65, (Ser547).
Epitope
C-terminus
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Bovine, Chimpanzee, Mouse, Porcine, Rat based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_068810
Gene Symbol
RELA
NFKB3
Modifications
Phosphorylation
Purification Method
Affinity Purfied
UniProt Number
Q04206
Molecular Weight
~62 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in p65 WT transfected U2OS cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho NFkB p65, (Ser547) in 10 µg of p65 WT transfected U2OS cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
