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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS543 |
| Description | Anti-Colipase Antibody |
| Alternate Names |
|
| Background Information | The protein Colipase is a co-enzyme needed for maximal enzyme activity of pancreatic lipase. It is a secreted protein produced by the pancreas that is originally secreted as an inactive proform, procolipase. Procolipase gets activated by cleavage by intestinal trypsin in the lumen of the small intestine. Once activated, Colipases function is to help overcome the inhibitory effect of bile salts on the lipase catalyzed hydrolysis of long-chain triglycerides that needs to occur within the duodenum. Colipase happens to have key hydrophobic amino acids that allow the complex of lipase and Colipase to adsorb to the lipid micelles, and this adsorption is essential for their digestion. Interestingly too, the procolipase propeptide also gives rise to the pentapeptide intestinal hormone Enterostatin, a small pentapeptide. Enterostatin acts to reduce food intake, in particular fat intake, when given to animals via injection or in to the brain. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Colipase Antibody | ABS543 is an antibody against Colipase Antibody for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Colipase in purified protein (DSilva, S., et Al. (2007). Journal of Lipid Research. 48:2478-2484). Western Blotting Analysis: A representative lot detected Colipase in mouse pancreas (Miller, R., et Al. (2009). AM J Physiol Endocrinol Metab. 297:E856-E865). Western Blotting Analysis: A representative lot detected Colipase in HEK293T cells transiently transfected to express Arg92 and Cys92 colipase (Xiao, X., et Al. (2013). Journal of Lipid Research. 54:514-521). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human Colipase. |
| Epitope | Unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~12 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse pancreas tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Colipase in mouse pancreas tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |