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Millipore This Anti-Ch25h Antibody Is Validated For Use In Western Blotting For The Detection Of Ch25h. - Mill (Additional S&H or Hazmat Fees May Apply)
CH25H , also known as Cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, h25OH, and encoded by the gene name CH25H, is a member of the sterol desaturase family. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol, which in turn leads to repression of the cholesterol biosynthetic enzymes. Recent studies have shown that CH25H may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. Other studies have also indicated that in testis, production of 25-hydroxycholesterol may play a role in Leydig cell differentiation.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-CH25H antibody is validated for use in Western Blotting for the detection of CH25H.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human CH25H near the N-terminus.
Epitope
Near N-terminus
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_003947
Gene Symbol
CH25H
Purification Method
Affinity Purfied
UniProt Number
O95992
Molecular Weight
~32 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in THP-1 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected CH25H in 10 µg of THP-1 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
