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Millipore This Anti-3-Pgdh Antibody Is Validated For Use In Western Blotting And Immunohistochemistry (Paraffin) For The Detection Of 3-Pgdh. - Mill (Additional S&H or Hazmat Fees May Apply)
3-PGDH (PHGDH) protein in an important enzyme in L-serine biosynthesis. 3-PGDH performs the first rate limiting step in the phosphorylated pathway of serine biosynthesis. 3-PGDH is also a biomarker in certain cancers that over express 3-PGDH. Moreover, 3-PGDH is the essential enzyme needed for cells to traverse the S phase and divide, and it plays a key role in T cell activation and proliferation for instance. Interestingly, even though 3-PGDH knockdown and reduced serine synthesis slows growth and proliferation of many cancer cells in vitro 3-PGDH knockdown does not affect tumor maintenance and growth in established breast cancer xenograft models, suggesting that 3-PGDH-dependent cancer cell growth may be context-dependent.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-3-PGDH Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of 3-PGDH.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected 3-PGDH in human cerebral cortex, human kidney, and human cerebellum tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human 3-PGDH near the N-terminus.
Epitope
Near N-terminus
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Mouse
Species Reactivity Note
Human, Mouse. Predicted to react with Porcine, Chimpanzee based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_006614
Gene Symbol
PHGDH
PGDH3
Purification Method
Affinity Purfied
UniProt Number
O43175
Molecular Weight
~54 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected 3-PGDH in 10 µg of A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
