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Millipore Anti-Mtor Antibody Is An Antibody Against Phospho-Mtor (Thr2164) For Use In DOT Blot, Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
FK506-binding protein 12-rapamycin complex-associated protein 1
FKBP12-rapamycin complex-associated protein
Mammalian target of rapamycin
mTOR
Mechanistic target of rapamycin
Rapamycin and FKBP12 target 1
Rapamycin target protein 1
Background Information
The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.
Anti-mTOR Antibody is an antibody against phospho-mTOR (Thr2164) for use in DOT Blot, Western Blotting.
Key Applications
DOT Blot
Western Blotting
Application Notes
Western Blot Analysis: A representative lot was used by an independent laboratory in HEK293 cell lysate. (Ekim, B., et Al. (2011). MCB. [Epub ahead of print].)
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human mTOR phosphorylated at Thr2164.
Epitope
Phosphorylated Thr2164
Host
Rabbit
Specificity
This antibody recognizes mTOR phosphorylated at Thr2164.
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_004949
Gene Symbol
MTOR
FRAP
FRAP1
FRAP2
RAFT1
RAPT1
Purification Method
Unpurified
UniProt Number
P42345
Molecular Weight
289 kDa calculated
Product Usage Statements
Quality Assurance
DOT Blot Specificity Analysis: Unmodified and phosphorylated mTOR peptides were probed with Anti-phospho-mTOR (Thr2164) (1:1,000 dilution).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
