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Intersectin-1 is a scaffolding protein facilitating clathrin-mediated endocytosis, vesicle trafficking and mitogen-activated protein kinase (MAPK) signaling pathways. Functional association studies of the protein have suggested ITSN1 has several differing roles in various processes such as RAS activation and stimulation of Cdc42 in order to accelerate actin assembly. Alternative splicing produces 4 isoforms. The major isoforms are ITSN1-L and ITSN1-S. Expression for ITSN1 is ubiquitous in adult/fetal tissue, except isoform 1 which is mostly found in the brain. The most notable expression is found in muscle, spleen, skeletal muscle, testis, heart, ovary and all fetal tissue. Additionly, the neurons of young Down Syndrome and pre-Alzheimer’s disease brains are predisposed for enlarged early endosomes which may contribute to these pathological processes.
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum with 0.05% sodium azide
Quality Level
MQ100
Applications
Application
Anti-Intersectin-1 is an antibody against Intersectin-1 for use in WB, ICC, IP.
Key Applications
Western Blotting
Immunocytochemistry
Immunoprecipitation
Application Notes
Immunocytochemistry Analysis: A representative lot of this antibody detected Intersectin-1 protein in HEK293T cells (Martin, N., et al. Mol Pharmacol 70:1643–1653, 2006) Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipiated Intersectin-1 (short and long isoforms) from mouse brain lysate (Martin, N., et al. Mol Pharmacol 70:1643–1653, 2006)
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the SH3 domain of Xenopus Intersectin-1.
Epitope
SH3 domain of Xenopus Intersectin-1 protein
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Rat
Mouse
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001080955
Gene Symbol
ITSN1
Purification Method
Unpurified
UniProt Number
O42287
Molecular Weight
~150 and ~200 kDa observed. Previous publication indicated the 200 kDa to be the long isoform and 150 kDa to be the short isoform (Okur, M., et al. Mol. Cell. Biol. 2014, 34(2):271). Uncharacterized bands may be detected at high antibody concentrations or longer exposures.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting on rat brain cytosol tissue lysate
Western Blotting Analysis: A 1:1000 dilution of this antibody detected Intersectin-1 protein in 10 µg of rat brain cytosol.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
