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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H | WB, ICC, IP | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS984 |
| Description | Anti-Intersectin-1 Antibody |
| Alternate Names |
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| Background Information | Intersectin-1 is a scaffolding protein facilitating clathrin-mediated endocytosis, vesicle trafficking and mitogen-activated protein kinase (MAPK) signaling pathways. Functional association studies of the protein have suggested ITSN1 has several differing roles in various processes such as RAS activation and stimulation of Cdc42 in order to accelerate actin assembly. Alternative splicing produces 4 isoforms. The major isoforms are ITSN1-L and ITSN1-S. Expression for ITSN1 is ubiquitous in adult/fetal tissue, except isoform 1 which is mostly found in the brain. The most notable expression is found in muscle, spleen, skeletal muscle, testis, heart, ovary and all fetal tissue. Additionly, the neurons of young Down Syndrome and pre-Alzheimer’s disease brains are predisposed for enlarged early endosomes which may contribute to these pathological processes. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal serum with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Intersectin-1 is an antibody against Intersectin-1 for use in WB, ICC, IP. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot of this antibody detected Intersectin-1 protein in HEK293T cells (Martin, N., et Al. Mol Pharmacol 70:1643–1653, 2006) Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipiated Intersectin-1 (short and long isoforms) from mouse brain lysate (Martin, N., et Al. Mol Pharmacol 70:1643–1653, 2006) |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to the SH3 domain of Xenopus Intersectin-1. |
| Epitope | SH3 domain of Xenopus Intersectin-1 protein |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~150 and ~200 kDa observed. Previous publication indicated the 200 kDa to be the long isoform and 150 kDa to be the short isoform (Okur, M., et Al. Mol. Cell. Biol. 2014, 34(2):271). Uncharacterized bands may be detected at high antibody concentrations or longer exposures. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting on rat brain cytosol tissue lysate Western Blotting Analysis: A 1:1000 dilution of this antibody detected Intersectin-1 protein in 10 µg of rat brain cytosol. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |