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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Mk | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS988 |
| Description | Anti-ACAT-2 Antibody |
| Alternate Names |
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| Background Information | Acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT-2), also known as Sterol O-acyltransferase 2 (SOAT2), is a synthetic enzyme that is important in lipoprotein assembly, steroid metabolism, and dietary cholesterol metabolism and absorption. ACAT-2 is expressed primarily in the liver and intestine in enterocytes in their endoplasmic reticulum. ACAT-2 esterifies cholesterol allowing it to be more efficiently packaged and transported. Free cholesterol can be taken up by lipoproteins but it is confined to the outer surface of the lipoparticle. Esterification allows internal packaging and much more efficient transport through the blood stream. Gene expression of ACAT-2 is partially regulated by the transcriptional repressor TGIF1 as well as other factors such as thyroid hormone expression and microRNA miR181d. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit Polyclonal in buffer containing 20 mm NaPO4, 28 mm NaCl and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-ACAT-2 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of ACAT-2. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected ACAT-2 in human liver tissue (Parini, P., et Al. (2004). Circulation. 2004;110:2017-2023). Western Blot Analysis: A representative lot detected ACAT-2 in human liver lysate (Parini, P., et Al. (2004). Circulation. 2004;110:2017-2023) |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to Human ACAT-2. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Antigen Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~ 55 kDa observed, uncharacterized bands may appear at lower molecular weight |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human lung tissue lysate using a non-reduced gel. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ACAT-2 in 10 µg of human lung tissue lysate using a non-reduced gel |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |