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FKBP-8, also known as Peptidyl-prolyl cis-trans isomerase PPIase FKBP8, FKBP8, 38 kDa FK506-binding protein, 38 kDa FKBP, FKBP-38, hFKBP38, FK506-binding protein 8, FKBP-8, FKBPR38, Rotamase, is encoded by the gene name FKBP8 and FKBP38. FKBP-8 is in constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Recent studies have shown that FKBP-8 may act as a chaperone for BCL2, by targeting BCL2 to the mitochondria and modulating its phosphorylation state. Furthermore, the BCL2/FKBP8/calmodulin/calcium complex may play a role in the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis. FKBP-8 is expressed widely, with highest levels detected in brain. FKBP-8 is also highly abundant in the retina.
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This FKBP-8 antibody is validated for use in WB, IH for the detection of FKBP-8.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Western Blotting Analysis: A representative lot detected FKBP-8 in HeLa transfected with myc-FKBP38 and HEK293 cell lysate (Zou, H., et al. (2013). JBC. 1-9). Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FKBP-8 in human cerebral cortex and human thalamus tissue.
Biological Information
Immunogen
Recombinant protein corresponding to the N-terminus of human FKBP-8.
Epitope
N-terminus
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_036313
Gene Symbol
FKBP8
FKBP38
Purification Method
Unpurified
UniProt Number
Q14318
Molecular Weight
~60 kDa observed. The calculated molecular weight is 44 kDa, however FKBP-8 has been shown at ~53-62 kDa most likely due to post-translational modification.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FKBP-8 in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
