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Six-transmembrane epithelial antigen of prostate 4
Tumor necrosis factor-alpha-induced adipose-related protein
STEAP4
Background Information
Metalloreductase STEAP4 (STEAP4), also known as Dudulin-4, Six-transmembrane epithelial antigen of prostate 4, Tumor necrosis factor-alpha-induced adipose related protein, and encoded by the gene name STEAP4 and TIARP, is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP4 inhibits anchorage-independent cell proliferation and uses NAD+ as acceptor. STEAP4 is associated with obesity and insulin-resistance, and might be involved in inflammatory arthritis, through the regulation of inflammatory cytokines. STEAP4 has been shown to play an important role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses. STEAP4 interacts with and phosphorylates PTK2/FAK1. STEAP4 is expressed in white and brown adipose tissues cells, as well as in muscle and liver cells. In addition, STEAP4 has been detected in joints and spleens of arthritic mice.
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-STEAP4 is an antibody against STEAP4 for use in WB, IH.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A representative lot detected STEAP4 in Adipose tissue, prostate tissue, and prostate cancer tissue (Freyhaus, H., et al. (2012) Cell Metabolism. 16:81-89).
Biological Information
Immunogen
Recombinant protein corresponding to mouse STEAP4.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_473439
Gene Symbol
Steap4
Tiarp
Purification Method
Unpurified
UniProt Number
Q923B6
Molecular Weight
~58 kDa observed. The calculated molecular weight is 52 kDa, however STEAP4 has been shown as a ~58 kDa band in some western blots (Freyhaus, H., et al. (2012) Cell Metabolism. 16:81-89).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse liver tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected STEAP4 in 10 µg of mouse liver tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
