Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT131 |
| Description | Anti-PTRF/cavin-1 Antibody |
| Alternate Names |
|
| Background Information | Polymerase I and transcript release factor (PTRF), also known as “cavin-1,” is a small cytoplasmic protein, which interacts with caveolin-1 to assemble specialized membrane invaginations (or caveolae), in various types of cells. Caveolae house specific proteins and lipids and play a central role in cell signaling and endocytosis. PTRF may also play a role in metabolism. Mice lacking functional PTRF are affected by hyperinsulinemia and glucose intolerance. In addition, PTRF may play a role in prostate cancer. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PTRF/cavin-1 Antibody detects level of PTRF/cavin-1 & has been published & validated for use in Western Blotting, IHC(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PTRF/cavin-1 in mouse cardiac tissue and mouse alveoli tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the Coiled Coil domain of human PTRF/cavin-1. |
| Epitope | Coiled Coil domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the Coiled Coil domain of PTRF/cavin-1. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human, Mouse, and Rat. Predicted to react with Bovine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| Molecular Weight | ~60 kDa, ~43 kDa observed. Three isoforms at 43 kDa, 33 kDa, and 23 kDa are known to exist. This protein was observed at ~60 kDa by an independent laboratory due to post-translational modification (Hill, M. M., et Al. (2008). Cell. 132(1):113-124.). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human lung tissue lysate. Western Blot Analysis: 1 mg/mL of this antibody detected PTRF/cavin-1 in 10 mg of human lung tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |