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Millipore Anti-L1cam Antibody Is A Highly Specific Rabbit Polyclonal Antibody, That Targets Neural Cell Adhesion Molecule L1 & Has Been Tested In Western Blotting & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules (N-CAM), myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts.
Product Information
Format
Affinity Purified
Control
Human brain tissue lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-L1CAM Antibody is a highly specific rabbit polyclonal antibody, that targets Neural cell adhesion molecule L1 & has been tested in western blotting & IHC (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Western Blotting Analysis: 0.4 µg/mL from a representative lot detected L1CAM in 10 µg of rat brain tissue lysate. Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected L1CAM in human kidney and human cerebellum tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the extracellular domain of human L1CAM.
Epitope
Extracellular domain
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the extracellular domain of L1CAM in addition antibody recognizes the full length form (~220 kDa) and the cleaved form (~140 kDa) of L1CAM.
Species Reactivity
Human
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_076493
Gene Symbol
L1CAM
CAML1
MIC5
Purification Method
Affinity Purfied
UniProt Number
P32004
Molecular Weight
~220 / 140 kDa observed. The full length protein and the cleaved form of L1CAM have been observed at ~220 kDa and ~140 kDa, respectively (Kalus, I., et al. (2003). The Journal of Biological Chemistry. 278(12):10381-10388). Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 0.4 µg/mL of this antibody detected L1CAM in 10 µg of human brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
