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Millipore This Girdin Antibody Is Validated For Use In Wb, Ihc, If & Ip For The Detection Of The Girdin Protein. - Mill (Additional S&H or Hazmat Fees May Apply)
Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.
Product Information
Format
Affinity Purified
Control
Cos-7 cell lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Girdin antibody is validated for use in WB, IHC, IF & IP for the detection of the Girdin protein.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Immunofluorescence
Immunoprecipitation
Application Notes
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Girdin in human colorectal cancer tissues.
Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa, Cos-7, and Att20 cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in HeLa cell lysate.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the coiled coil domain rat Girdin.
Epitope
Coiled coil domain.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the coiled-coil domain of Girdin.
Species Reactivity
Human
Monkey
Rat
Species Reactivity Note
Demonstrated to react with Human and Monkey. Predicted to react with Rat based upon immunogen design. Other homologies: Mouse (97% sequence homology).
Antibody Type
Polyclonal Antibody
Entrez Gene Number
XP_223709
Purification Method
Affinity Purfied
Molecular Weight
~220 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Cos-7 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Girdin in 10 µg of Cos-7 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
