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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT180 |
| Description | Anti-CDCP1 Antibody |
| Alternate Names |
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| Background Information | CUB domain-containing protein 1 (CDCP1) is also called Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein (SIMA135), Transmembrane and associated with src kinases and CD_antigen 318. CDCP1 is part of the tetraspanin web involved in tumor progression and metastasis. CDCP1 may also be involved in cell adhesion, cell matrix association and regulation of anchorage versus migration or proliferation versus differentiation via phosphorylation status. CDCP1 is a possible marker for leukemia diagnosis, and for subsets of immature hematopoietic stem cell. CDCP1 is highly expressed in mitotic cells, skeletal muscle, colon, many human tumor cell lines, colorectal cancer, breast carcinoma and lung cancer. CDCP1 may also be found in cells phenotypically similar to mesenchymal stem cells and neural stem cells. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | Anti-CDCP1 Antibody is a highly specific rabbit polyclonal antibody, that targets CUB domain-containing protein 1 & has been tested in western blotting & IHC (Paraffin). |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 2 µg/mL of this antibody detected CDCP1 in a panel of human tumor tissue lysates. Immunohistochemistry Analysis: A 1:100 and a 1:500 dilution from a representative lot detected CDCP1 in human breast carcinoma sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human CDCP1. |
| Epitope | Cytoplasmic domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the cytoplasmic domain of CDCP1. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Other Homologies: Mouse and Rat (77% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~135 kDa and ~70 kDa observed. Uniprot describes 3 isoforms at 92 kDa, 72 kDa, and 38 kDa, due to alternative splicing. The full length and cleaved form of CDCP1 have been observed at ~135 kDa and ~70 kDa (Casar, B., et al. (2012). Oncogene 31(35):3924-3938.). Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human pancreas tumor tissue lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected CDCP1 in 10 µg of human pancreas tumor tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |