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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT49 |
| Description | Anti-Dynamin-2 Antibody |
| Alternate Names |
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| Background Information | Dynamin-2 is a ubiquitously expressed, membrane associated protein belonging to the Dynamin protein family of GTPases, a group consisting of three isoforms with distinct tissue specificity and function. Dynamin localizes to plasma membrane-coated pits by its interactions with amphiphysin through the dynamin PRD domain. Dynamin-2 is a component in the manufacture of microtubule bundles, and is capable of both binding and hydrolyzing GTP. It is also likely to be involved in the trafficking mechanisms for vesicles, especially those pertaining to endocytosis. Defects in the expression of Dynamin-2 have been shown to cause Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB), and centronuclear myopathy autosomal dominant (ADCNM). There is also some interest in Dynamin-2 as a potential regulator for adenovirus targeting cancer therapies. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit polyclonal serum containing 0.5% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Dynamin-2 using this Anti-Dynamin-2 Antibody validated for use in WB. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Dynamin-2. |
| Host | Rabbit |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis. CATALYTIC ACTIVITY: GTP + H2O = GDP + phosphate. SUBUNIT STRUCTURE: Interacts with MYOF By similarity. Interacts with SHANK1, SHANK2, SH3BP4 and NOSTRIN. SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytoskeleton. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell junction › synapse. Note: Microtubule-associated. Also found in the postsynaptic density of neuronal cells. TISSUE SPECIFICTY: Ubiquitously expressed. INVOLVEMENT IN DISEASE: Defects in DNM2 are a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies (CNMs) are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. CNMs comprise a wide spectrum of phenotypes, ranging from severe neonatal to mild late-onset familial forms. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) [MIM:606482]. Charcot-Marie-Tooth disease (CMT) is a clinically and genetically heterogeneous disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. CMTDIB is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. MISCELLANEOUS: Overexpression of CNM- and CMT-related DNM2 mutants in COS7 cells, whatever the mutated domain, led to a reduction in clathrin-mediated receptor endocytosis associated with MAPK ERK-1 and ERK-2 impairment. The membrane trafficking impairment process may represent a common pathophysiological pathway in the autosomal forms of CNM DNM2-CMT neuropathy. SEQUENCE SIMILARITIES: Belongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain. |
| Molecular Weight | ~ 98 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 1:1,000 dilution of the antibody detected Dynamin-2 in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |