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Coiled-coil domain-containing protein 53 (CCDC53) is one of several components comprising the WASH complex, a complex localized to endosomal surfaces that functions to upregulate actin polymerization through the recruiting and activation of Arp2/3 complex. WASH is a critical component in the fission of tubules (transport intermediates involved in the distribution of endosomes). The WASH complex is composed of CCDC53, F-actin-capping protein subunits alpha and beta, WASH, FAM21, KIAA1033, and KIAA196.
Product Information
Format
Purified
Control
HepG2 cell lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-CCDC53 Antibody is validated for use in WB for the detection of CCDC53.
Key Applications
Western Blotting
Application Notes
Western Blot Analysis: 2.5 µg/mL from a representative lot detected CCDC53 in 10 µg of mouse brain and PC12 cell lysate.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human CCDC53.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Human
Mouse
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_057137
Gene Symbol
CCDC53
CGI-116
Purification Method
Protein G Purified
UniProt Number
Q9Y3C0
UniProt Summary
FUNCTION: Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting.
SUBUNIT STRUCTURE: Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53.
SEQUENCE SIMILARITIES: Belongs to the CCDC53 family.
Molecular Weight
~28 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: 2.5 µg/mL of this antibody detected CCDC53 in 10 µg of HepG2 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
