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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT70 |
| Description | Anti-WASH complex subunit 7 (SWIP) Antibody |
| Alternate Names |
|
| Background Information | WASH complex subunit 7 (SWIP) is a component of the WASH complex belonging to the WASH S7 family. The WASH complex is localized at early endosomal subdomains involved in the activation and recruitment the ARP 2/3 complex in order to stimulate actin polymerization and tubulin interaction. Through its interaction with FAM21, WASH plays a regulatory role in the retromer-mediated trafficking of vesicles. It is also involved in the tubule fission, components required in endosomal sorting processes as transport intermediates. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect WASH complex subunit 7 (SWIP) using this Anti-WASH complex subunit 7 (SWIP) Antibody validated for use in IP. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human WASH complex subunit 7. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting By similarity. SUBUNIT STRUCTURE: Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. SEQUENCE SIMILARITIES: Belongs to the WASHS7 family. SEQUENCE CAUTION: The sequence AAI10851.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence BAA82985.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. |
| Molecular Weight | ~135 kDa observed. IgG heavy chain may be observed at approximately 50 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunoprecipitation/Western Blot Analysis in HeLa immunoprecipitation with rabbit IgG and Anti-WASH complex subunit 7. Immunoprecipitation/Western Blot Analysis: 5 µg/mL of this antibody detected WASH complex subunit 7 in 10 µg of HeLa immunoprecipitation with rabbit IgG and Anti-WASH complex subunit 7. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |