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| Description | |
|---|---|
| Catalogue Number | AC111P |
| Replaces | AP300P |
| Description | Sheep Anti-Mouse IgG Antibody, Species absorbed Antibody, HRP conjugate |
| Background Information | Immunoglobulin g (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fc receptors which interact with sialylated Fc fragments on IgG molecules. |
| Product Information | |
|---|---|
| Format | HRP |
| Presentation | Purified sheep polyclonal antibody in PBS with StabilZyme® HRP Conjugate Stabilizer and 0.05% Proclin. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate, Cat. No. AC111P, is a secondary antibody HRP conjugate, validated for use in Western Blotting application with little or no cross-reactivity toward mouse IgA & IgM, chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: Performance of a representative lot was validated at a 1:4,000 dilution for target band labeling following a mouse monoclonal GAPDH antibody (Cat. No. MAB374) in Western blotting application using 10 µg of HeLa cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | Purified mouse IgG. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Sheep |
| Specificity | Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG2>IgG2a>IgG3>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM. |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reaction toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species. |
| Antibody Type | Polyclonal Antibody |
| Purification Method | Ammonium Sulfate. |
| Molecular Weight | ~150.0 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: Performance of this HRP-conjugated secondary antibody (Cat No. AC111P) is validated at a 1:4,000 dilution for target band labeling following a mouse monoclonal GAPDH antibody (Cat. No. MAB374) in Western blotting application using 10 µg of A431 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 500 µL |