| Description | |
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| Catalogue Number | AG909 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | ADAM 17, control peptide for AB19027 |
| Overview | The peptide is used for blocking the activity of anti-TACE, C-Terminal (Catalog number AB19027). Incubate the peptide with equal volume of antibody for 30 minutes at 37oC to block the activity of anti-TACE in Western blotting. Amino Acids 807 to 823 (ASFKLQRQNRVDSKETE) of human TACE (Black et Al. 1997; Moss et Al. 1997). |
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| Product Information | |
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| Presentation | Liquid in PBS containing 0.1% BSA and 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
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| Key Applications |
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| Application Notes | For use in blocking the reactivity of AB19027. Optimal working dilution must be determined by the end user. |
| Biological Information | |
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| Concentration | 200 μg/mL |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P78536 # Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor and the amyloid precursor protein. Also involved in the activation of Notch pathway (By similarity). COFACTOR: Binds 1 zinc ion per subunit. SIZE: 824 amino acids; 93021 Da SUBUNIT: Interacts with MAD2L1. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney. DOMAIN: SwissProt: P78536 Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR (By similarity). & The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. PTM: The precursor is cleaved by a furin endopeptidase (By similarity). & Phosphorylated. Stimulation by growth factor or phorbol 12- myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. SIMILARITY: Contains 1 disintegrin domain. & Contains 1 peptidase M12B domain. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
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| Material Size | 50 µg |