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| Description | |
|---|---|
| Catalogue Number | AP112A |
| Replaces | MABN1055 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Goat Anti-Human IgG Antibody, Alkaline Phosphatase conjugate |
| Overview | Alkaline Phosphatase-conjugated Affinity Purified Goat anti-Human IgG (H+L) |
| Product Information | |
|---|---|
| Format | Alkaline Phosphatase |
| Presentation | Lyophilized. Buffer = 0.01 M Tirs HCl, 0.25 M NaCl, pH 8.0 with 15 mg/mL BSA and 0.05% sodium azide. RECONSTITUTION: Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Human IgG using this Goat anti-Human IgG Antibody, Alkaline Phosphatase conjugate validated for use in ELISA & WB. |
| Key Applications |
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| Application Notes | EIA and Western blots: 1:5,000-1:50,000. Immunohistochemistry: 1:1,000-1:2,000. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Host | Goat |
| Specificity | Based on immunoelectrophoresis, the antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but the antibody may cross-react with immunoglobulins from other species. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Purification Method | Serum |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade for better) to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. WARNING: For research use only; not for use as a diagnostic. |
| Packaging Information | |
|---|---|
| Material Size | 1 mL |