Special Offers
100% Performance Guaranteed
| Description | |
|---|---|
| Catalogue Number | AP124F |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Goat Anti-Mouse IgG Antibody, (H+L) FITC Conjugated |
| Background Information | Immunoglobulin g (IgG) is a multimeric immunoglobulin, built of two heavy chains γ and two light chains. Each complex has two antigen binding sites. It is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. Furthermore, this is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| Product Information | |
|---|---|
| Format | FITC |
| Presentation | Lyophilized from 0.02 M phosphate buffered saline, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide and 15 mg/mL bovine serum albumin. F/P Ratio: 14.5 μg FITC per mg of antibody |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Goat anti-Mouse IgG Antibody, (H+L) FITC Conjugated is an antibody against Mouse IgG for use in IF. |
| Key Applications |
|
| Application Notes | Affinity purified antibody shows a single band against Rabbit anti-Goat Serum when run as the antigen in IEP at a minimum concentration of 10 mg/mL. Suggested dilution for most applications: 1:50-1:200. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Concentration | 2 mg/mL (after reconstitution) |
| Host | Goat |
| Specificity | Affinity purified antibody shows a single band when run in an IEP system at a minimum concentration of 10 mg/mL using whole mouse serum as the antigen. Purity: Affinity purified antibody shows a single band against Rabbit anti Goat Serum when run as the antigen in IEP at a minimum concentration of 10 mg/mL. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Purification Method | ImmunoAffinity Purified |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Affinity purified antibody shows a single band against Rabbit anti Goat Serum when run as the antigen in IEP at a minimum concentration of 10 mg/mL. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized product at 2-8°C for up to 12 months after date of receipt. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. Protect from light. |
| Packaging Information | |
|---|---|
| Material Size | 2 mg |