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Millipore Donkey Anti-Rabbit Igg Antibody, Biotin-Sp Conjugate, Species Adsorbed Detects Level Of Donkey Rabbit Igg & Has Been Published & Validated For Use In ELISA & Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
Donkey Anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed
Background Information
Immunoglobulin g (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Product Information
Format
Biotin
Presentation
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.15 M NaCl, pH 7.1 with 15 mg/mL BSA, and 0.1% sodium azide.
RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.
Quality Level
MQ100
Applications
Application
Donkey anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed detects level of Donkey Rabbit IgG & has been published & validated for use in ELISA & WB.
Key Applications
ELISA
Western Blotting
Application Notes
EIA: 1:50,000-1:100,000 Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000. Immunohistochemistry: 1:500-1:5,000 Flow cytometry: 1:200-1:1,000 Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000 Optimal working dilutions must be determined by end user.
Biological Information
Host
Donkey
Specificity
Rabbit IgG
Species Reactivity
Rabbit
Antibody Type
Polyclonal Antibody
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
